Introduction to Sequence Analysis
Interpreting the results of BLAST2 EVEC
¥Underneath
the score list, you can view the pairwise alignments of sequences from the
score list against your query sequence. Note that matching sequences are
connected with a "|" symbol. As this is a perfect match, all of
the nucleotides are connected with a "|" symbol, mismatches would be
connected with a blank space. A gap would be represented with a "-"
symbol. Thus a sequence alignment can be represented in the format:
Score= normalized bits (raw
bits)
The score of the alignment, called score or bit
score, is the sum of log odds scores of each matching amino acid pair
in the alignment less gap penalites; the raw score in bits is also shown in
parentheses.
The expect value E (E-value) of chance matches of
unrelated sequences from a database of this size and the percent identities in
the alignment is shown.