4
Introduction to Sequence Analysis
Nucleotide Analysis
Checking for vector contamination
¥ DNA/RNA from a biological source are usually inserted into a cloning vector (e.g. Plasmid or phage) so that they can be cloned.
¥ Sequencing of such constructs frequently produces raw sequences that include segments derived from vectors.
¥ Also transposable elements from the cloning host (generally bacteria or yeast) can insert into the cloned DNA/RNA while the clone is being propagated and will then be sequenced, as can DNA/RNA contaminants.