¥ Click the button ÒBLAST
resultÓ to see the results
¥
¥ You should have noticed that
sequence 1 and sequence 2 appear to be contaminated with vectors
¥
¥ A closer look is required to see if these are genuine hits, or a
result of finding a loose match to a similar functional domain to one found in
the vectors database. This is due to the contents of the database, and the
e-values allowing these hits to pass through
¥
¥ In the case of sequence 1, you will
see that the parameter ÒValueÓ for all these hits is zero, so all
these hits are exact matches and are definitly a result of vector contamination
(no gaps in the alignment)
¥
¥ In the case of sequence 2, you will
see that the parameter ÒValueÓ for all these hits is always positive. As a rule of
thumb, for a first look at these, if the value is not Òto the
power ofÓ (e.g. 1.2x10-7) it is not likely to be significant
¥