19
Introduction to Sequence Analysis
Nucleotide Analysis
Running a NCBI-BLAST2 similarity search
¥BLAST stands for Basic Local Alignment Search tool.
¥It is aimed to find regions of sequence similarity, which will yield functional and evolutionary cluess about the structure and function of your query sequence.
¥We will now be able to find out what sequence Sequence 2 is, as this sequence is a real entry in the EMBL Nucleotide Sequence Database [EMBL release], and see what sequences it is in fact similar to.

We are going to use the form:
http://www.ebi.ac.uk/Tools/blastall/nucleotide.html















Sequence 2:
http://ubio.bioinfo.cnio.es/people/ograna/public_html/introductionToSequenceAnalysis/checkingForVectorContamination/