13
Introduction to Sequence Analysis
Nucleotide Analysis
Interpreting the results of BLAST2 EVEC
¥Lambda and K are statistical parameters used in calculating the significance of the gapped alignment score. They are determined on the basis of the alignment scores of random sequences using a combination of scoring matrix and gap penalties (Bioinformatics, D. W Mount, page 251).
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¥H is the relative entropy that shows the ability of the entire substitution matrix to discriminate related from unrelated sequences.
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¥The scoring matrix that was used
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¥The gap creation/gap extension costs
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¥Values for various steps in the search process leading to the identification of HSPs (High-scoring element pair).
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¥Calculated values for database size, query size, and so on used in the search.