¥ Click the
button ÒBLAST resultÓ to see the results
¥ You should
have noticed that sequence 1 and sequence 2 appear to be contaminated with
vectors
¥ A closer look is
required to see if these are genuine hits, or a result of finding a loose match
to a similar functional domain to one found in the vectors database. This is
due to the contents of the database, and the e-values allowing these hits to
pass through
¥ In the
case of sequence 1, you will see that the parameter ÒValueÓ for all
these hits is zero, so all these hits are exact matches and are definitly a
result of vector contamination (no gaps in the alignment)
¥ In the
case of sequence 2, you will see that the parameter ÒValueÓ for all
these hits is always positive. As a rule of thumb, for a first look at these,
if the value is not Òto the power ofÓ (e.g. 1.2x10-7) it is not
likely to be significant